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2H2SO4=692
Sensitive to -ß-lactamase
Cold Spring Harbour laboratory
coli strains are provided by LB Broth
Yeast extract 10 Peptone 20 Maltose 20 Agar 20 Suspend 70 gram powdered medium in 1 litre distilled water
HL5-C Medium without Glucose supplemented with vitamins and micro elements Size:6 x 1kg 2H2SO4=692HL5 C supplemented with vitamins and micro elements as present in FM and SIH media. The addition of extra vitamins and micro elements gives good results for cultures of Dicty who require additional nutritional elements for starting up vigorous growth or protein synthesis. Component g l Peptone 5 Yeast Extract 5 Tryptone 5 KH2PO4 1. 2 Na2HPO4 0. 35 FM Vitamins and Micro elements 0. 01 Suspend 16. 55 gram powdered medium in 1 litre distilled water.
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