FM drop out mixture, minus Lysine, 7400 mg/l Size:250g AX Medium is used to

AX Medium is used to induce and to cultivate an axenic culture of Dictyostelium discoideum from bacterially grown cells

0005% Pb (Lead) <0

Tryptone 35 Yeast Extract 20 NaCl 5 Agar 15 Final pH 7

Dilute 50 ml MES-SDS 20X into 950 ml of deionised water to make 1 litre of MES-SDS Running Buffer

49 Agar 17 Suspend 24

FM drop out mixture, minus Lysine, 7400 mg/l Size:250g AX Medium is used toThe FM drop out mixture, minus Lysine, is based on the Amino acids present in FM Minimal medium. These Amino acids form a basis for many Drop Out mixtures to select for auxotrophic requirements and transformants. Each FM Drop Out mixture contain all components except for one or two essential Amino acids, i. e. the dropped out supplements. FM Amino acids drop out mixtures are used in combination with FM Minimal medium w o Amino acids or FM Minimal