HL5 Medium including Glucose supplemented with vitamins and micro elements Size:6 x 1kg The hydroxyl group present at

The hydroxyl group present at the 3 position of G-418 is phosphorylated by NPTII

By omitting Potassium DiHydrogen Phosphate (KH2PO4)

Dithiothreitol C4H10O2S2=154

6  g/L Store at room temperature

Complete Supplement Mixture formulations are available in 25 gram and 250 gram pack sizes

HL5 Medium including Glucose supplemented with vitamins and micro elements Size:6 x 1kg The hydroxyl group present atHL5 supplemented with vitamins and micro elements as present in FM and SIH Media. The addition of extra vitamins and micro elements gives good results for cultures of Dicty who require additional nutritional elements for starting up vigorous growth or protein synthesis Component g l Peptone 14 Yeast Extract 7 Glucose 13. 5 KH2PO4 0. 5 Na2HPO4 0. 5 FM Vitamins and Micro elements 0. 01 Suspend 35. 5 gram powdered medium in 1 litre distilled water. Store