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Tryptone 8 Yeast extract 5 NaCl 5 Agar 7 Final pH 7
6 L-Tyrosine 36
resulting in an increase of cell density up to 5 x 10^7
L-Broth is a nutritionally rich medium originally developed for growth and maintenance of recombinant E
C6H12O6 180
Vegetal LoFlo Medium Size:6 x 1kg Tryptone 8 Yeast extract 5LoFlo vs VLoFlo: Triplicate biological repeats (cultures) Cell counts made to 48 h Note: by 26 h cells round and shrink* in both LoFlo and VLoFlo By 47 h cell rounding and shrinkage pronounced and assay stopped * Noted previously in Dicty community that LoFlo is not ideal for long term culturing. Typically, cells are grown only overnight in LoFlo, and transferred to fresh Lo Flo the following morning prior to imaging, so that LoFlo growth limited to
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